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Donor information on the human fibroblasts (HFs). M: Man; Y: Year.

Journal: Cells

Article Title: From Young to Old: Mimicking Neuronal Aging in Directly Converted Neurons from Young Donors

doi: 10.3390/cells13151260

Figure Lengend Snippet: Donor information on the human fibroblasts (HFs). M: Man; Y: Year.

Article Snippet: The primary human fibroblasts (HFs) and the corresponding directly converted neurons (iNs) were either obtained from Takara Bio (Kusatsu, Shiga, Japan) or were provided by Dr. Zameel Cader (University of Oxford) and predictive toxicology (StemBANCC) consortium.

Techniques:

Experimental timeline. HFs carrying the ‘all-in-one’ vector UNA are converted to iNs over 3–4 weeks. The iNs were sorted using fluorescence-activated cell sorting (FACS) to obtain a pure population of immature neurons and then replated. After 72 h, the stress approaches were started. Abbreviations: HFs: human fibroblasts; iNs: directly converted neurons.

Journal: Cells

Article Title: From Young to Old: Mimicking Neuronal Aging in Directly Converted Neurons from Young Donors

doi: 10.3390/cells13151260

Figure Lengend Snippet: Experimental timeline. HFs carrying the ‘all-in-one’ vector UNA are converted to iNs over 3–4 weeks. The iNs were sorted using fluorescence-activated cell sorting (FACS) to obtain a pure population of immature neurons and then replated. After 72 h, the stress approaches were started. Abbreviations: HFs: human fibroblasts; iNs: directly converted neurons.

Article Snippet: The primary human fibroblasts (HFs) and the corresponding directly converted neurons (iNs) were either obtained from Takara Bio (Kusatsu, Shiga, Japan) or were provided by Dr. Zameel Cader (University of Oxford) and predictive toxicology (StemBANCC) consortium.

Techniques: Plasmid Preparation, Fluorescence, FACS